Zeiss Lattice Light Sheet (LLS)
Instrument description
The Lattice Light-Sheet microscope is an advanced system for high-resolution live-cell imaging, minimizing phototoxicity and photobleaching while achieving near-isotropic resolution (~250 nm in X, Y, and Z). It enables fast volumetric imaging (up to three volumes per second), allowing real-time observation of dynamic subcellular processes. The system uses light-sheet illumination with a lattice of interfering Bessel beams, producing a thin and uniform sheet that improves axial resolution and reduces out-of-focus light. An integrated incubation system controls temperature, humidity, and CO₂, enabling long-term, unsupervised live imaging experiments.
Access procedures
Internal users (CNR / affiliated institutions)
Access is available through the institutional intranet portal: [intranet link].
Instrument use must be booked in advance and approved by the instrument manager (elisa.deluca@cnr.it)
External users
External access is possible under the following agreements:
1.Scientific collaboration – use of the instrument within a joint research project or bilateral collaboration agreement.
2.Training – hands-on sessions supervised by qualified laboratory staff.
3.Use under technical assistance
4.Service – measurement and analysis performed by the facility staff on samples provided by the user
Indicative fees
Scientific collaboration
To be defined
Training and self use
Includes technical support and independent use of the instrument with on demand technical support.
Technical supervision
To be defined
Service
Hourly instrument fee: 150-250 € analytical fee: 150-300 €
Elyra 7
Instrument description
The ELYRA 7 is a fluorescence microscope based on Structured Illumination Microscopy (SIM), including advanced Lattice SIM and SIM² reconstruction, achieving ~120 nm lateral resolution. It also functions as a conventional brightfield and fluorescence microscope and uses two sCMOS cameras for simultaneous high-speed acquisition. An integrated incubation system controls temperature, humidity, and CO₂, enabling long-term, unsupervised live imaging experiments. The system integrates STORM and PALM techniques, also in TIRF mode, enabling super-resolution down to 20–30 nm through single-molecule localization. These approaches allow nanoscale analysis of biological structures, such as protein localization and membrane organization.
Access procedures
Internal users (CNR / affiliated institutions)
Access is available through the institutional intranet portal: [intranet link].
Instrument use must be booked in advance and approved by the instrument manager (elisa.deluca@cnr.it)
External users
External access is possible under the following agreements:
1.Scientific collaboration – use of the instrument within a joint research project or bilateral collaboration agreement.
2.Training – hands-on sessions supervised by qualified laboratory staff.
3.Use under technical assistance
4.Service – measurement and analysis performed by the facility staff on samples provided by the user
Indicative fees
Scientific collaboration
Based on the specific research agreement or collaboration framework.
To be defined
Training and self use
Includes technical support and independent use of the ELYRA 7 with on-demand assistance
Hourly user fee: 75-110 €
Hourly instrument fee: 50-80 €
Technical supervision
To be defined
