CytoFLEX S (Beckman-Coulter)
Instrument description
Benchtop flow cytometer equipped with 4 lasers (1 violet for nanomaterial acquisition) for the simultaneous acquisition of 9 fluorescence parameters, as well as 2 physical parameters (polychromatic analysis). Equipped with an automatic acquisition system for reading 96-well plates for high-throughput analysis. The instrument is available for research activities, training sessions, and service-based analyses within scientific collaborations and supported projects.
Access procedures
Internal users (CNR / affiliated institutions)
Access is available through the institutional intranet portal: [intranet link].
Instrument use must be booked in advance and approved by the instrument manager (ilariaelena.palama@cnr.it)
External users
External access is possible under the following agreements:
1.Scientific collaboration – use of the instrument within a joint research project or bilateral collaboration agreement.
2.Training – hands-on sessions supervised by qualified laboratory staff.
3.Use under technical assistance
4.Service – measurement and analysis performed by the facility staff on samples provided by the user
Indicative fees
Scientific collaboration
To be defined
Training and self use
Includes technical support and independent use of the instrument with on demand technical support.
Technical supervision
To be defined
Service
CytoFLEX SRT (Beckman-Coulter)
Instrument description
High-performance cell separator, equipped with 4 lasers (1 violet for nanomaterial acquisition), allows the simultaneous analysis of 15 fluorescence parameters and 2 physical parameters. It is used to physically separate up to 4 different cell populations simultaneously, based on multidimensional phenotypic and/or functional analyses. The separated cell populations (up to 100% pure) are viable and can be cultured and/or used for subsequent genomic-functional analyses.
Access procedures
Internal users (CNR / affiliated institutions)
Access is available through the institutional intranet portal: [intranet link].
Instrument use must be booked in advance and approved by the instrument manager (ilariaelena.palama@cnr.it)
External users
External access is possible under the following agreements:
1.Scientific collaboration – use of the instrument within a joint research project or bilateral collaboration agreement.
2.Training – hands-on sessions supervised by qualified laboratory staff.
3.Use under technical assistance
4.Service – measurement and analysis performed by the facility staff on samples provided by the user
Indicative fees
Scientific collaboration
To be defined
Training and self use
Includes technical support and independent use of the instrument with on demand technical support.
Hourly user fee: 90 €
Hourly instrument fee: 75 €
Technical supervision
To be defined
Service
Hourly instrument fee: 180 € analytical fee: 150 €
CyTOF XT + Hyperion XTi (Standard Biootools)
Instrument description
high-performance mass cytometer that identifies single cells labeled with stable metal isotope-labeled antibodies, allowing measurement of both phenotypic and functional markers. It simultaneously detects over 50 markers per cell and works on both pelleted and suspended samples.
Access procedures
Internal users (CNR / affiliated institutions)
Access is available through the institutional intranet portal: [intranet link].
Instrument use must be booked in advance and approved by the instrument manager (ilariaelena.palama@cnr.it)
External users
External access is possible under the following agreements:
1.Scientific collaboration – use of the instrument within a joint research project or bilateral collaboration agreement.
2.Training – hands-on sessions supervised by qualified laboratory staff.
3.Use under technical assistance
4.Service – measurement and analysis performed by the facility staff on samples provided by the user
Indicative fees
Scientific collaboration
To be defined
Training and self use
Includes technical support and independent use of the instrument with on demand technical support.
Hourly user fee: 75 €
Hourly instrument fee: 95 €
Technical supervision
To be defined
Service
Hourly instrument fee: 75 € analytical fee: 95 €
